One-third of singers and vocal professionals report experiencing a benefit from empirical vitamin B₁₂ injections for improvement of mild singing-related symptoms (eg, reduced stamina, vocal fatigue, and effort).

However, there is no objective evidence to support or refute these claims.

We assess the presence and magnitude of the effect of empirical vitamin B₁₂ injection on the vocal performance of singers.

A randomized, double-blind, placebo-controlled, crossover trial was conducted from November 7, 2017, to November 30, 2018, at an academic voice center among 20 active adult singers without dysphonia but with mild vocal symptoms.

Individuals with known or suspected vitamin B₁₂ deficiency or active or recent vitamin B₁₂ treatment were excluded. Analysis was on a per-protocol basis.